Tuesday, January 17, 2012

Glycolysis?

The of fructose 1,6 bisphosphate by aldolase b generates two 3 carbon fragments which are inequilibriul via triose isomerase.(dihydroxyacetone -phosphate to glycerol-phosphate, and glyceraldehyde -3 phosphate). As glyceraldehyde -3 P is used in the next step and converted to 1, 3 bisphosphoglycerate..then more dHAP shifts toward glyceraldehyde -3P because of LeChatelier's principle ( the delta G for the reaction is very close to zero ) . So theoretically you have two glyceraldehyde-3 Ps instead of one so all reactions past aldolase one doubles the yields...This is why 2 ATPs are invested ( hexokinase and phosphofructokinase ) but 4 are made ( post aldolase)for a net yield of 2 ATPs. The phosphate donor is ATP the 6 phosphate is part of fructose-6P made frpm glucose -6-P which came from action of hexokinase on glucose .The rate limting enzyme of glycolysis , phosphofructokinase uses another ATP to phosphorylate fructose 6-P to the 1, 6 bis P

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